Thursday, 30 May 2013

EFFECT OF PH ON THE RESPIRATION

EFFECT OF PH ON THE RESPIRATION

The pH value of this research report on freshwater bivalves
Mollusks, Indonaia cyan from Pima River, Pandharpur nearby. Freshwater
Mollusks are considered aquatic ecosystems most important component. This is
As Lincoln is also involved in the food chain. Distributed in a large number of bivalve molluscs
Various land waters, and is considered an ecosystem biological indicators. Any
Changing the surrounding environment, has internal physiological bivalve molluscs.
Pandharpur is one of the famous pilgrimage center, attracting millions of believers
Various yatras. Human interventions affect the water quality of the river Bhima. In
This study attempts to understand the effects of changes has been the main
Breathing bivalve molluscs, Indonaia cyan. Experiment
15 days interval of 5 for 15 days respiration rate analysis
As a control experimental group. Oxygen consumption rate changes are:
Compared with controls, and that disturbed physiological adjustments. Result
As discussed in the pH change rate of the concentration of keywords physiological changes:
pH, respiration, cyan Indonaia,: Pima River.
Introduction:

Many biotic and abiotic factors affecting bivalve molluscs respiration rate (Bain, 1976).
Domestic and industrial wastewater can change a variety of physical and chemical parameters of aquatic bodies. On
Parameters are pH and attention because it is the industrial discharges into the ocean it'simportant
Environment has been studied by the (Perking.1976). Vedhapathak. Et al (2011) studied the rate of oxygen
Consumption, ammonia excretion advertisement O: N ratio of freshwater bivalves Indonaia cyan Godavari
River Paithan. effect of pH very little work has been reported in accordance with the rate of change
Breathing rivers from India, especially from the Pima heavily polluted river from the west
Maharashtra. Therefore, this investigation has undertaken to keep in view the importance and
Rich freshwater mollusks, cyan Indonaia from Pima River and the impact of changes in pH
Effects on respiration were studied.
Materials and Methods:

Freshwater bivalves, Indonaia blue river banks collect from Pima Gopalpur site (latitude 17O 66'N 75 ° 36 'east longitude), in November and December 2012.The animals
There, the size of 5864 mm, select test shortly after collection. The animals
Back to the lab and clean removal of algal biomass advertising mud. Ago
Experimental animals were acclimatized under laboratory conditions one week. And grouping
Divided into three batches, each containing 30 hatch. The first to be deemed to control the pH 7.4
And the second batch of bivalve molluscs containing 30 animals exposed to pH 6.0 (acidic). Third with 30
Animals are exposed to a pH of 8.0 (basic). I think considering the control batch and batch II and III
As an experiment. Bivalve molluscs carried out 15 days (chronic effect of pH
Effect). Interval 5thand 15 天 control group and experimental respiratory rate determined
Water is changed during group experimentationto duration of 12-130 hours. In order to obtain an acidic pH, 20% 1N
The use of hydrochloric acid, and the obtained alkaline pH, using 40% of 1N NaOH. PH value
Water holding the duration of the experiment animals confirmed the digital pH meter (ELECO
Hyderabad). Rates of individual animals from the control and experimental O2consumption
According to the modified Wrinklers determined, the specific use of freshwater (Golterman. etc.
1978). For the determination O2consumption, a total of five individual animals from each group were used (control group,
a pH of 6, pH value 8) and O2consumption individual animals in each group was measured and the average
And standard deviation values ​​were determined from each of the groups that the oxygen consumption. To
Confirmation, and statistical analysis, triplicate readings is oxygen
Estimates. Criteria for the determination of oxygen consumption of one liter bottle breathing
Use siphon inlet and outlet. In order to determine the oxygen consumption of individual bivalve molluscs,
indonaia cyan, oxygen content were measured in the beginning of the experiment, after one hour
Animals exposed to a liter tank containing breathing apparatus tightness test.
Five separate bivalve from the average of each group statistical analysis to be used for
Know the great value, Results and Discussion:

The change rate of the oxygen consumption of the experimental results shown in
Following the table in Figure no.1and One
Used in the experiment of the physical and chemical parameters of the water temperature
18.22oc, pH value of 7.2-7.8, the oxygen content of 5.8-6.2 mg / l, total hardness of 105-110 ppm. Rate of oxygen
After spending the first five days, the control group was 0.0700Â ± 0.0046 O2/lit/gm/hr's. In the experiment
Groups reduced to 70.14%, a pH value of 6.0 (acidic), and at a pH of 8.0 (base) in the experimental group, which is
Decreased by 46.14%. On the other hand, the control group of the experiment after 15 days, the rate of oxygen
Consumption O20.0471Â ± 0.0019 mg / g / l / hr. After 15 days in the experimental group at pH 6.0
(Acidic) fell 30.57%, pH value 8.0 (basic) O2 content of 29.51%, reducing the time
With the respective control group. From our findings, in general, it can be said that after five days of
Experimental bivalve acidic and alkaline significantly reduced significantly
Oxygen consumption rate. Acidic groups decreased respiration rate, but also highlights
Basic group. However, the decreasing pattern still maintain 15 days after the change
At pH (acidity and alkalinity) noted that in bivalve molluscs. This clearly shows that changing any one of
Physical-chemical parameters (pH value) will certainly affect the physiological status of bivalve molluscs
River Pima Pandharpur's. change in pH animal metabolic rate can be changed, because the
Acidic and basic groups because the pressure imbalance. May change due to respiratory metabolism
Overall weakened animals. Universal Polites (1980) studied the oxygen absorption and
Transportation molluscs prosobranch, Busycon conalicultum gas exchange and response
Hypoxia. thurberg, et al, (1974) noted that any alternate metabolic rate may be a path
Showed biochemical composition may involve compensatory mechanism
Various stress conditions. Oxygen consumption is considered to be two metabolic rate as an indicator, as well as
Indicates the status of the stress conditions (Cantelemo, 1977) of the organism. In the present study, it can be
Said decrease in oxygen consumption may be due to stress conditions, as change in pH
This effect may be due to substantially the target organs, such as gills, may be responsible for reducing the
Oxygen consumption. Siddaki (2010) observed oxygen consumption declined from fish, Clarius
Toad, let them come into contact with the metal stress (copper sulfate), Shelke Wani (2005) observed
From: freshwater Amblypharyngodonm overturned after expressing their oxygen consumption
Stress and that various metals, gill damage can reduce the impact of the metal
The oxygen absorption efficiency of the organism. This may be the same as this investigation,
Bivalves Indonaia blue changed pH (acid and alkaline) may have been caused by the stress exceeds the gills, and then change has reduced the metabolic rate of oxygen consumption. However, further
Investigation and respiratory metabolism with special requirements in endonuclease analysis concerns
Reference freshwater bivalve molluscs, after Indonaia cyan induced changes in acid and alkali
Balance to make final conclusions Acknowledgements:

The authors thank Academy of Arts and Sciences, Solapur Walchand principle for its
Necessary laboratory facilities. : Respiration freshwater bivalve molluscs, Indonaia cyan impact of changes in the pH,
(Values ​​in parentheses represent percentage difference compared with controls).
* Denotes significant at P pH of this research report on freshwater bivalves
Mollusks, Indonaia cyan from Pima River, Pandharpur nearby. Freshwater
Mollusks are considered aquatic ecosystems most important component. This is
As Lincoln is also involved in the food chain. Distributed in a large number of bivalve molluscs
Various land waters, and is considered an ecosystem biological indicators. Any
Changing the surrounding environment, has internal physiological bivalve molluscs.
Pandharpur is one of the famous pilgrimage center, attracting millions of believers
Various yatras. Human interventions affect the water quality of the river Bhima. In
This study attempts to understand the effects of changes has been the main
Breathing bivalve molluscs, Indonaia cyan. Experiment
15 days interval of 5 for 15 days respiration rate analysis
As a control experimental group. Oxygen consumption rate changes are:
Compared with controls, and that disturbed physiological adjustments. Result
Discussed in the physiological changes due to changes in pH, the concentration ratio.

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